E-ISSN 2756-4053 | ISSN 2705-3156
 

Research Article 


EXPRESSION OF THE RECOMBINANT HUMAN EXONUCLEASE1 (hExo1) USING BACULOVIRUS EXPRESSION SYSTEM

Umar AA, Sani I and Aminu H.

Abstract
Human exonuclease1 (hExo1) acts directly in diverse DNA processing events, including replication, mismatch repair (MMR) and double strand break repair (DSBR). It was also recently described to function as damage sensor and apoptosis inducer when DNA damage
occur. The aim of this study is to express the recombinant hExo1 protein from insect cells. Recombinant hExo1 protein was functionally expressed in insect High Five™ cells by the BAC-TO-BAC®baculovirus expression system. Recombinant hExo1 was purified to near homogeneity by affinity chromatography using heparin column. A yield of about 200 μg of the hExo1 protein was purified from 500 mL of infected High Five™ cells. The purified protein was analysed by SDS/PAGE, which revealed that the hExo1 protein was isolated in its monomeric form.

Key words: hExo1, DNA, Expression, Insect cells, Double strand break


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Umar AA
Articles by Sani I and Aminu H
on Google
on Google Scholar

How to Cite this Article
Pubmed Style

AA U, H SIaA. EXPRESSION OF THE RECOMBINANT HUMAN EXONUCLEASE1 (hExo1) USING BACULOVIRUS EXPRESSION SYSTEM. JIRLS. 2019; 1(1): 76-81.


Web Style

AA U, H SIaA. EXPRESSION OF THE RECOMBINANT HUMAN EXONUCLEASE1 (hExo1) USING BACULOVIRUS EXPRESSION SYSTEM. https://www.jirlsonline.com/?mno=108404 [Access: April 08, 2022].


AMA (American Medical Association) Style

AA U, H SIaA. EXPRESSION OF THE RECOMBINANT HUMAN EXONUCLEASE1 (hExo1) USING BACULOVIRUS EXPRESSION SYSTEM. JIRLS. 2019; 1(1): 76-81.



Vancouver/ICMJE Style

AA U, H SIaA. EXPRESSION OF THE RECOMBINANT HUMAN EXONUCLEASE1 (hExo1) USING BACULOVIRUS EXPRESSION SYSTEM. JIRLS. (2019), [cited April 08, 2022]; 1(1): 76-81.



Harvard Style

AA, U. & H, . S. I. a. A. (2019) EXPRESSION OF THE RECOMBINANT HUMAN EXONUCLEASE1 (hExo1) USING BACULOVIRUS EXPRESSION SYSTEM. JIRLS, 1 (1), 76-81.



Turabian Style

AA, Umar, and Sani I and Aminu H. 2019. EXPRESSION OF THE RECOMBINANT HUMAN EXONUCLEASE1 (hExo1) USING BACULOVIRUS EXPRESSION SYSTEM. Journal of Innovative Research in Life Sciences, 1 (1), 76-81.



Chicago Style

AA, Umar, and Sani I and Aminu H. "EXPRESSION OF THE RECOMBINANT HUMAN EXONUCLEASE1 (hExo1) USING BACULOVIRUS EXPRESSION SYSTEM." Journal of Innovative Research in Life Sciences 1 (2019), 76-81.



MLA (The Modern Language Association) Style

AA, Umar, and Sani I and Aminu H. "EXPRESSION OF THE RECOMBINANT HUMAN EXONUCLEASE1 (hExo1) USING BACULOVIRUS EXPRESSION SYSTEM." Journal of Innovative Research in Life Sciences 1.1 (2019), 76-81. Print.



APA (American Psychological Association) Style

AA, U. & H, . S. I. a. A. (2019) EXPRESSION OF THE RECOMBINANT HUMAN EXONUCLEASE1 (hExo1) USING BACULOVIRUS EXPRESSION SYSTEM. Journal of Innovative Research in Life Sciences, 1 (1), 76-81.