E-ISSN 2756-4053 | ISSN 2705-3156
 

Research Article 


IN VITRO AND IN VIVO ANTICOAGULANT EFFECT OF METHANOL EXTRACT OF Manihot esculenta ROOT TUBER (EUPHORBIACEAE) IN RAT MODELS

Umaru M.L., Onum E., Etuk E.U., Uyaiabasi G.N., Tijjani R.G., Zayyanu U., Jega A.Y., Alebiosu C.O.

Abstract
Defects in the natural mechanisms of coagulation system resulting in thrombus formation in blood vessels are the third most common cause of death and disability globally. Anticoagulants and thrombolytics available are associated with different adverse effect and subsequent complications. This study attempts to evaluate the anticoagulation effect of the methanol extract of Manihot esculenta tubers (MEMET) using Albino rat models. Qualitative phytochemical screening and acute toxicity studies were conducted using standard procedures. The in vitro thrombolytic model was used to conduct assay on activated partial thromboplastin time (APTT), prothrombin time (PT) and Clot lysis (CL) while in the in vivo assay, bleeding time (BT) and clotting time (CT) were evaluated. Streptokinase, heparin and distilled water were used as controls, respectively. The phytochemical screening of the MEMET revealed the presences of carbohydrates, saponins, tannins, phenols, cardiac glycosides, triterpenoids and alkaloids however, flavonoids, diterpenes and proteins were found to be absent in this study. Oral acute toxicity study gave an LD50 value of >2000 mg/kg. The MEMET at the highest dose of 625mg/kg showed significant (p<0.05) increase in APTT, PT, CL, BT and CT when compared with the groups administered with distilled water as negative control. Based on the findings in this work, MEMET contain properties that inhibit coagulation and thrombosis and could be a potential natural source of anticoagulant and thrombolytic agent.

Key words: Blood coagulation, Manihot esculenta, Cassava, Bleeding time, Clotting time


 
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How to Cite this Article
Pubmed Style

Umaru M.L., Onum E., Etuk E.U., Uyaiabasi G.N., Tijjani R.G., Zayyanu U., Jega A.Y., Alebiosu C.O. IN VITRO AND IN VIVO ANTICOAGULANT EFFECT OF METHANOL EXTRACT OF Manihot esculenta ROOT TUBER (EUPHORBIACEAE) IN RAT MODELS. JIRLS. 2023; 5(1): 1-10.


Web Style

Umaru M.L., Onum E., Etuk E.U., Uyaiabasi G.N., Tijjani R.G., Zayyanu U., Jega A.Y., Alebiosu C.O. IN VITRO AND IN VIVO ANTICOAGULANT EFFECT OF METHANOL EXTRACT OF Manihot esculenta ROOT TUBER (EUPHORBIACEAE) IN RAT MODELS. https://www.jirlsonline.com/?mno=183198 [Access: January 07, 2024].


AMA (American Medical Association) Style

Umaru M.L., Onum E., Etuk E.U., Uyaiabasi G.N., Tijjani R.G., Zayyanu U., Jega A.Y., Alebiosu C.O. IN VITRO AND IN VIVO ANTICOAGULANT EFFECT OF METHANOL EXTRACT OF Manihot esculenta ROOT TUBER (EUPHORBIACEAE) IN RAT MODELS. JIRLS. 2023; 5(1): 1-10.



Vancouver/ICMJE Style

Umaru M.L., Onum E., Etuk E.U., Uyaiabasi G.N., Tijjani R.G., Zayyanu U., Jega A.Y., Alebiosu C.O. IN VITRO AND IN VIVO ANTICOAGULANT EFFECT OF METHANOL EXTRACT OF Manihot esculenta ROOT TUBER (EUPHORBIACEAE) IN RAT MODELS. JIRLS. (2023), [cited January 07, 2024]; 5(1): 1-10.



Harvard Style

Umaru M.L., Onum E., Etuk E.U., Uyaiabasi G.N., Tijjani R.G., Zayyanu U., Jega A.Y., Alebiosu C.O (2023) IN VITRO AND IN VIVO ANTICOAGULANT EFFECT OF METHANOL EXTRACT OF Manihot esculenta ROOT TUBER (EUPHORBIACEAE) IN RAT MODELS. JIRLS, 5 (1), 1-10.



Turabian Style

Umaru M.L., Onum E., Etuk E.U., Uyaiabasi G.N., Tijjani R.G., Zayyanu U., Jega A.Y., Alebiosu C.O. 2023. IN VITRO AND IN VIVO ANTICOAGULANT EFFECT OF METHANOL EXTRACT OF Manihot esculenta ROOT TUBER (EUPHORBIACEAE) IN RAT MODELS. Journal of Innovative Research in Life Sciences, 5 (1), 1-10.



Chicago Style

Umaru M.L., Onum E., Etuk E.U., Uyaiabasi G.N., Tijjani R.G., Zayyanu U., Jega A.Y., Alebiosu C.O. "IN VITRO AND IN VIVO ANTICOAGULANT EFFECT OF METHANOL EXTRACT OF Manihot esculenta ROOT TUBER (EUPHORBIACEAE) IN RAT MODELS." Journal of Innovative Research in Life Sciences 5 (2023), 1-10.



MLA (The Modern Language Association) Style

Umaru M.L., Onum E., Etuk E.U., Uyaiabasi G.N., Tijjani R.G., Zayyanu U., Jega A.Y., Alebiosu C.O. "IN VITRO AND IN VIVO ANTICOAGULANT EFFECT OF METHANOL EXTRACT OF Manihot esculenta ROOT TUBER (EUPHORBIACEAE) IN RAT MODELS." Journal of Innovative Research in Life Sciences 5.1 (2023), 1-10. Print.



APA (American Psychological Association) Style

Umaru M.L., Onum E., Etuk E.U., Uyaiabasi G.N., Tijjani R.G., Zayyanu U., Jega A.Y., Alebiosu C.O (2023) IN VITRO AND IN VIVO ANTICOAGULANT EFFECT OF METHANOL EXTRACT OF Manihot esculenta ROOT TUBER (EUPHORBIACEAE) IN RAT MODELS. Journal of Innovative Research in Life Sciences, 5 (1), 1-10.